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SibEnzyme Ltd. :: Display product information: Aco I
Aco I
Catalog numberE499E500
Package, u.a.100500
Concentration, u.a./ml500-2000500-2000

Recognition siteY^GGCCR
Methylation sensitivityBlocked by overlapping dcm methylation (CmCWGG): CCTGGCCR.
OriginAcinetobacter calcoaceticus
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA(dam-, dcm-)
LigationAfter 3-fold overdigestion with AcoI more than 90% of Lambda DNA fragments can be ligated with T4 DNA Ligase at 16C and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of AcoI for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
50 - 7510050 - 7525 - 5075 - 100

References: Chernukhin, V.A., Belichenko, O.A., Tomilova, J.E., Dedkov, V.S., Doroganov, A.O.. Unpublished observations. (2006)