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SibEnzyme Ltd. :: Display product information: BstSF I
Name
BstSF I
Catalog numberE197E198
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteC^TRYAG
GAYRT^C
Methylation sensitivitynot tested
OriginBacillus stearothermophilus SF
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutesNo
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10025 - 5010050 - 7550 - 75

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).