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SibEnzyme Ltd. :: Display product information: Rga I
Rga I
Catalog numberE491E492
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteGCGAT^CGC
Methylation sensitivityNot blocked by overlapping Dam-methylation (GmATC): GCGATCGC
OriginRhizoblum galegae
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onAdenovirus-2 DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10050 - 7510 - 2525 - 50100

Note: High enzyme concentration may result in star activity.