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SibEnzyme Ltd. :: Display product information: Mal I
Name
Mal I
Catalog numberE489E490
Package, u.a.50250
Concentration, u.a./ml500-1000500-1000

Recognition siteG(mA)^TC
CT^(mA)G
Methylation sensitivitynot tested
OriginMarinococus albus I
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA, 50% glycerol; Store at -20C.
Assayed onpBR322 DNA (dam-methylated)
LigationAfter 2-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of DNA with 2 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferSE-buffer MalI (20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5025 - 5050 - 7575 - 10050 - 75

References: