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SibEnzyme Ltd. :: Display product information: BstNS I
Name
BstNS I
Catalog numberE251E252
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteRCATG^Y
Y^GTACR
Methylation sensitivitynot tested
OriginBacillus stearothermophilus NS
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10050 - 7510 - 2510 - 2575 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1996).