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SibEnzyme Ltd. :: Display product information: Bmu I
Name
Bmu I
Catalog numberE487E488
Package, u.a.100500
Concentration, u.a./ml500-1000500-1000

Recognition siteACTGGG(N)5^
TGACCC(N)4^
Methylation sensitivitynot tested
OriginBacillus megaterium S87
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C
Assayed onLambda DNA (HindIII-digest)
LigationAfter 2-fold overdigestion with enzyme about 75% of the DNA fragments can be ligated and 95% may be recut.
Non-specific activityOvernight digest with BmuI is not recommended. A 50 μl reaction containing 1 μg of Lambda DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10075 - 10025 - 5010 - 25100

Note: Enzyme is active in presence of EDTA.