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SibEnzyme Ltd. :: Display product information: Mnl I
Mnl I
Catalog numberE481E482
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteCCTC(N)7^
Methylation sensitivityBlocked by overlapping CG methylation: CCTmCG.
OriginE.coli strain, that carries the cloned gene Mnl I from Moraxella nonliquefaciens
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 50% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
75 - 10010025 - 5025 - 5075 - 100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Brinkley, P., Bautista, D.S., Graham, F.L.; Gene 100: 267-268 (1991).