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SibEnzyme Ltd. :: Display product information: BstMB I
Catalog numberE119E120
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition site^GATC
Methylation sensitivityBlocked by overlapping Dam methylation(GmATC): GATC .
Not blocked by CG methylation.
Not cut hemimethylated site: 5- G(6mA)TC-3 / 3-CTAG-5
OriginBacillus stearothermophillus MB
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 ug/ml BSA; . Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65C
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
10 - 2525 - 5010075 - 10010 - 25

References: Kovalenko, K., Kileva, E.V., Mezentseva, N.V., Mikhnenkova, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations. (2004)