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SibEnzyme Ltd. :: Display product information: BslF I
BslF I
Catalog numberE479E480
Package, u.a.100500
Concentration, u.a./ml10001000

Recognition siteGGGAC(N)10^
Methylation sensitivitynot tested
OriginBacillus stearothermophilus Fl
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 90% of DNA fragments can be ligated and 95% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
25 - 5025 - 5010 - 2525 - 50100

Note: High enzyme concentration may result in star activity.
Long incubation with BSA is not recommend.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
There is DNA-methyltransferase activity in presence of SAM. It is maximum at 48C. In presence of 10mM MgCl2 enzyme both modifies and hydrolyzes DNA. If MgCl2 is absent enzyme modifies DNA only. And that DNA become proof against BslFI.
BslF I also cleaves the sequence GGGAC(11/15).
References: Nadeev, A.N., Kashirina, J.G., Nayakshina, T.N., Dedkov, V.S., Mezentseva, N.V., Tomilova, J.E., Degtyarev, S.K.; Unpublished observations. 2004