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SibEnzyme Ltd. :: Display product information: PspX I
Name
PspX I
Catalog numberE477E478E478X
Package, u.a.20010001000
Concentration, u.a./ml100001000050000

Recognition siteVC^TCGAGB
BGAGCT^CV
Methylation sensitivitynot tested
OriginE.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2 -mercaptoethanol, 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7550 - 7525 - 5075 - 100100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.

References:

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