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SibEnzyme Ltd. :: Display product information: BstMC I
Catalog numberE071E072
Package, u.a.5002500
Concentration, u.a./ml50005000

Recognition siteCGRY^CG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus MC
Storage conditions10 mM Tris-HCl(pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes80oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
10075 - 10010 - 2510 - 2550 - 75

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml
Do not use BSA for long incubation.
References: Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).