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SibEnzyme Ltd. :: Display product information: PspC I
Name
PspC I
Catalog numberE475E476
Package, u.a.200010000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteCAC^GTG
GTG^CAC
Methylation sensitivitynot tested
OriginPseudomonas species C
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20ºC. Store at -70ºC is recommended for period longer than 30 days.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Ligation is better in presence of 10% PEG.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
10050 - 750 - 100 - 1050 - 75

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Tronin, A., Kileva, E.V., Mezentseva, N., Dedkov, V.S., Degtyarev, S.K. Unpublished observations. (2003)