|Catalog number||E475||E476 |
|Package, u.a.||2000||10000 |
|Concentration, u.a./ml||10000-30000||10000-30000 |
|Methylation sensitivity||not tested|
|Origin||Pseudomonas species C|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20ºC. Store at -70ºC is recommended for period longer than 30 days.|
|Assayed on||Lambda DNA|
|Ligation||After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Ligation is better in presence of 10% PEG.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA|
Enzyme % activity:
|100||50 - 75||0 - 10||0 - 10||50 - 75|
Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Tronin, A., Kileva, E.V., Mezentseva, N., Dedkov, V.S., Degtyarev, S.K.
Unpublished observations. (2003)