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SibEnzyme Ltd. :: Display product information: Mbo II
Mbo II
Catalog numberE471E472
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteGAAGA(N)8^
Methylation sensitivityBlocked by overlappin dam-methylation(GmATC): GAAGATC.
OriginE.coli strain, that carries the cloned gene Mbo II from Moraxella bovis
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 5-fold overdigestion with enzyme more than 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG ligation is better.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10075 - 10025 - 5050 - 75100

References: Brown N.L., Hutchison C.A. III, Smith M. J. Mol. Biol. 140: 143-148 (1980).