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SibEnzyme Ltd. :: Display product information: Ajn I
Name
Ajn I
Catalog numberE473E474
Package, u.a.2001000
Concentration, u.a./ml500-3000500-3000

Recognition site^CCWGG
GGWCC^
Methylation sensitivityNot blocked by dcm methylation: CCWGG
OriginAcinetobacter johnsonii R2
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 6 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5010 - 2510 - 2525 - 50100

Note: At 370C activity is about 10% from maximum.

References:

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