Go to sibenzyme.com
SibEnzyme Ltd. :: Display product information: Zrm I
Name
Zrm I
Catalog numberE005E006
Package, u.a.6003000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteAGT^ACT
TCA^TGA
Methylation sensitivitynot tested
OriginZoogloea ramigera SCA
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7525 - 500 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Nadeev, A.N., Kileva, E.V., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2003)