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SibEnzyme Ltd. :: Display product information: BstH2 I
Name
BstH2 I
Catalog numberE171E172
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition siteRGCGC^Y
Y^CGCGR
Methylation sensitivitynot tested
OriginBacillus stearothermophilus H2
Storage conditions10 mM KPO4(pH 7.2); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7550 - 750 - 1010 - 25100

Note: High enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1994).