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SibEnzyme Ltd. :: Display product information: BstFN I
Name
BstFN I
Catalog numberE283E284
Package, u.a.3001500
Concentration, u.a./ml2000-100002000-10000

Recognition siteCG^CG
GC^GC
Methylation sensitivityBlocked by CG methylation
OriginBacillus stearothermophilus FN
Storage conditions20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 10 mM MgCl2; 200 ug/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10050 - 7525 - 5025 - 50100

References: Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1999).