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Name BstF5 I Catalog number E031 E032 Package, u.a. 500 2500 Concentration, u.a./ml 10000 10000 Recognition site GGATGNN^ CCTAC^NN Methylation sensitivity not tested Origin Bacillus stearothermophilus F5 Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C. Optimum temperature 65 oC Inactivation 20 minutes 80oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme % activity: B G O W Y 75 - 100 50 - 75 25 - 50 50 - 75 100 Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,25. References: Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Gene 172: 49-51 (1996).