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SibEnzyme Ltd. :: Display product information: BstF5 I
Name
BstF5 I
Catalog numberE031E032
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition siteGGATGNN^
CCTAC^NN
Methylation sensitivitynot tested
OriginBacillus stearothermophilus F5
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; and 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10050 - 7525 - 5050 - 75100

Note: The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,25.
References: Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Gene 172: 49-51 (1996).