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SibEnzyme Ltd. :: Display product information: BstDE I
Catalog numberE227E228
Package, u.a.5002500
Concentration, u.a./ml10000-5000010000-50000

Recognition siteC^TNAG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus DE
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 30-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 60 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
75 - 10010025 - 5050 - 7510 - 25

Note: At 37C activity is 10% from maximum
References: Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).