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SibEnzyme Ltd. :: Display product information: BstAP I
Catalog numberE259E260
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteGCANNNN^NTGC
Methylation sensitivitynot tested
OriginBacillus stearothermophilus AP
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 ug/ml BSA; and 50% glycerol Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
25 - 5025 - 5075 - 10010025 - 50

Note: High enzyme concentration may result in star activity. At 37C activiti is 50% from maximum.
The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
References: Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Nucleic Acids Res. 25: 2301-2302 (1997).