|Catalog number||E259||E260 |
|Package, u.a.||200||1000 |
|Concentration, u.a./ml||5000||5000 |
|Methylation sensitivity||not tested|
|Origin||Bacillus stearothermophilus AP|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 ug/ml BSA; and 50% glycerol Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.|
|Optimum temperature||60 oC|
|Inactivation 20 minutes||80oC|
|Optimum SE-buffer||W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)|
Enzyme % activity:
|25 - 50||25 - 50||75 - 100||100||25 - 50|
Note: High enzyme concentration may result in star activity. At 37°C activiti is 50% from maximum.
The minimum number of units that resulted in complete digestion of 1 ug of substrate DNA in 16 hours is 0,5.
References: Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Nucleic Acids Res. 25: 2301-2302 (1997).