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SibEnzyme Ltd. :: Display product information: Bst4C I
Bst4C I
Catalog numberE265E266
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteACN^GT
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 4C
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme less than 10% of DNA fragments can be ligated.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
75 - 10075 - 10010 - 2525 - 50100

References: Shinkarenko, N.M., Schevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1997).