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SibEnzyme Ltd. :: Display product information: Bst2U I
Bst2U I
Catalog numberE051E052
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition siteCC^WGG
Methylation sensitivityNot blocked by overlapping Dcm methylation(CmCWGG): CCWGG.
OriginBacillus stearothermophilus 2U
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter Bst2UI overdigestion none of DNA fragmetns can be ligated.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
75 - 10010050 - 7550 - 7510 - 25

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.

References: Myakisheva, T.V., Degtyarev, S.K. Unpublished observations (1995).