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Name Bst2U I Catalog number E051 E052 Package, u.a. 1000 5000 Concentration, u.a./ml 10000-20000 10000-20000 Recognition site CC^WGG GGW^CC Methylation sensitivity Not blocked by overlapping Dcm methylation(CmCWGG): CCWGG. Origin Bacillus stearothermophilus 2U Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After Bst2UI overdigestion none of DNA fragmetns can be ligated. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60°C. Optimum temperature 60 oC Inactivation 20 minutes 80oC Optimum SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA Enzyme % activity: B G O W Y 75 - 100 100 50 - 75 50 - 75 10 - 25 Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml. Do not use BSA for long incubation. References: Myakisheva, T.V., Degtyarev, S.K. Unpublished observations (1995).