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SibEnzyme Ltd. :: Display product information: Acc65 I
Name
Acc65 I
Catalog numberE003E004
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteG^GTACC
CCATG^G
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): GGTACCWGG.
OriginAcinetobacter calcoaceticus 65
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA(dcm-)
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5075 - 10010010 - 25

References: Prichodko, G.G., Rechkunova, N.I., Repin, V.E., Degtyarev, S.Kh., Sib. Biol. J. 1: 59-60 (1991).