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SibEnzyme Ltd. :: Display product information: Bst2B I
Name
Bst2B I
Catalog numberE043E044
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteC^TCGTG
GAGCA^C
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 2B
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10025 - 5010 - 2525 - 50100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Belichenko, O.A., Degtyarev, S.K. Unpublished observations (1995).