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SibEnzyme Ltd. :: Display product information: Bst6 I
Name
Bst6 I
Catalog numberE239E240
Package, u.a.2001000
Concentration, u.a./ml1000-50001000-5000

Recognition siteCTCTTC(N)1^
GAGAAG(N)4^
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 6
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C. Store at -70ºC is recommended for period longer than 30 days.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme 80% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10075 - 10050 - 7575 - 100100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000)