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SibEnzyme Ltd. :: Display product information: BssT1 I
Name
BssT1 I
Catalog numberE207E208
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteC^CWWGG
GGWWC^C
Methylation sensitivitynot tested
OriginBacillus stearothermophilus T1
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5025 - 5075 - 10010 - 25

Note: High enzyme concentration may result in star activity
References: Serov, G.D., Tereshenko, T.A., Puchkova, L.I., Rechkunova N.I., Degtyarev, S.K., Unpublished observations.