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SibEnzyme Ltd. :: Display product information: BssNA I
Name
BssNA I
Catalog numberE261E262
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition siteGTA^TAC
CAT^ATG
Methylation sensitivitynot tested
OriginBacillus stearothermophilus NA
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of DNA pBR322 fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutesNo
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
50 - 7550 - 7575 - 10010075 - 100

Note: High enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1996).