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SibEnzyme Ltd. :: Display product information: Bsp1720 I
Name
Bsp1720 I
Catalog numberE185E186
Package, u.a.100500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteGC^TNAGC
CGANT^CG
Methylation sensitivitynot tested
OriginBacillus species 1720
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Non-specific activityNo nonspecific activity was detected after incubation of 1ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7510050 - 7550 - 7575 - 100

References: Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).