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SibEnzyme Ltd. :: Display product information: Bsp19 I
Bsp19 I
Catalog numberE047E048
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteC^CATGG
Methylation sensitivitynot tested
OriginBacillus species 19
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2;; 200 mM NaCl; 1 mM DTT) + BSA

Enzyme % activity:
0 - 1010 - 2550 - 7575 - 10010 - 25

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations. Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M. Biotekhnologiya 0: 18-27 (1998).