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SibEnzyme Ltd. :: Display product information: Bsp13 I
Name
Bsp13 I
Catalog numberE183E184
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteT^CCGGA
AGGCC^T
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC): TCCGGATC and GATCCGGA.
OriginBacillus species 13
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5050 - 7575 - 10050 - 750 - 10

References: Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.