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SibEnzyme Ltd. :: Display product information: Acc36 I
Name
Acc36 I
Catalog numberE289E290
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition siteACCTGC(N)4^
TGGACG(N)8^
Methylation sensitivitynot tested
OriginAcinetobacter calcoaceticus 36
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
25 - 5025 - 5050 - 7550 - 75100

Note: High enzyme concentration may result in star activity
References: Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations. 1999