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SibEnzyme Ltd. :: Display product information: Bso31 I
Name
Bso31 I
Catalog numberE285E286
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition siteGGTCTC(N)1^
CCAGAG(N)5^
Methylation sensitivityNot blocked by methylation GGTCTmC
OriginBacillus stearothermophilus 31
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onT7 DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of T7 DNA with 5 u.a. of enzyme for 16 hours at 55C.
Optimum temperature55 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5075 - 10010075 - 10025 - 50

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Shinkarenko, N.M., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1999).