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SibEnzyme Ltd. :: Display product information: BseX3 I
Name
BseX3 I
Catalog numberE263E264
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteC^GGCCG
GCCGG^C
Methylation sensitivityBlocked by CG methylation.
OriginBacillus stearothermophilus X3
Storage conditions10 mM Tris-HCl (pH 8.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5010050 - 7510 - 25

References: Kileva, E.V., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1996).