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SibEnzyme Ltd. :: Display product information: BseP I
Name
BseP I
Catalog numberE181E182
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition siteG^CGCGC
CGCGC^G
Methylation sensitivityBlocked by CG methylation.
OriginBacillus stearothermophilus P
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50C.
Optimum temperature50 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7510075 - 10050 - 7550 - 75

References: Langdale, J.A., Myers, P.A., Roberts, R.J. Unpublished observations (1992).