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SibEnzyme Ltd. :: Display product information: Bse118 I
Name
Bse118 I
Catalog numberE039E040
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteR^CCGGY
YGGCC^R
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 118
Storage conditions10 mM KPO4 (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 2-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 2 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1050 - 7510075 - 10025 - 50

References: Repin, V.E., Babkin, I.V., Tereshenko, T.A. Bioorg. Khim. 19: 406-409 (1993).