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SibEnzyme Ltd. :: Display product information: Bse21 I
Bse21 I
Catalog numberE037E038
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition siteCC^TNAGG
Methylation sensitivitynot tested
OriginBacillus species 21
Storage conditions10 mM KPO4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (HindIII-digest)
LigationAfter 2-fold overdigestion with enzyme about 50% of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10% PEG. Of these more than 90% can be recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
50 - 7550 - 7510 - 2525 - 50100

References: Degtyarev, S.Kh., Rechkunova, N.I., Repin, V.E., Kolyhalov, A.A., Netesov, S.V. Izv. Sib. Otd. Akad. Nauk SSSR 1: 138-139 (1990).