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SibEnzyme Ltd. :: Display product information: Bse8 I
Bse8 I
Catalog numberE147E148
Package, u.a.10005000
Concentration, u.a./ml50005000

Recognition siteGATNN^NNATC
Methylation sensitivitynot tested
OriginBacillus species 8
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated and recut.
Non-specific activityLong incubation not recommended owing to occurrence of star activity
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
25 - 5010075 - 10075 - 10050 - 75

Note: High enzyme concentration (more than 5-fold overdigestion on 1 ug substrate) may result in star activity.
References: Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E. Bioorg. Khim. 19: 583-585 (1993).