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SibEnzyme Ltd. :: Display product information: Bse3D I
Name
Bse3D I
Catalog numberE253E254
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition siteGCAATGNN^
CGTTAC^NN
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 3D
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 60C.
Optimum temperature60 oC
Inactivation 20 minutes80oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2510025 - 5050 - 7575 - 100

References: Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (1996).