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SibEnzyme Ltd. :: Display product information: Bse1 I
Name
Bse1 I
Catalog numberE035E036
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteACTGGN^
TGAC^CN
Methylation sensitivitynot tested
OriginBacillus stearothermophilus 1
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65C.
Optimum temperature65 oC
Inactivation 20 minutes80oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10075 - 10025 - 5010 - 25100

References: Repin, V.E., Degtyarev, S.Kh. Unpublished observations.