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SibEnzyme Ltd. :: Display product information: Bsa29 I
Name
Bsa29 I
Catalog numberE205E206
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition siteAT^CGAT
TAGC^TA
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC): GATCGATC.
Blocked by CG methylation.
OriginBacillus stearothermophilus 29
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5010050 - 7550 - 7575 - 100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Repin, V.E., Degtyarev, S.K. Unpublished observations.