|Catalog number||E205||E206 |
|Package, u.a.||1000||5000 |
|Concentration, u.a./ml||20000||20000 |
|Methylation sensitivity||Blocked by overlapping Dam methylation (GmATC): GATCGATC.|
Blocked by CG methylation.
|Origin||Bacillus stearothermophilus 29|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA (dam-)|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|25 - 50||100||50 - 75||50 - 75||75 - 100|
Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 ug/ml.
Do not use BSA for long incubation.
References: Repin, V.E., Degtyarev, S.K.