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SibEnzyme Ltd. :: Display product information: Acc16 I
Name
Acc16 I
Catalog numberE001E002
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition siteTGC^GCA
ACG^CGT
Methylation sensitivitynot tested
OriginAcinetobacter calcoaceticus 16
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7575 - 10025 - 5010075 - 100

References: Degtyarev, S.Kh., Repin, V.E. unpublished observations. 1992