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SibEnzyme Ltd. :: Display product information: Bpu14 I
Name
Bpu14 I
Catalog numberE033E034
Package, u.a.10005000
Concentration, u.a./ml1000010000

Recognition siteTT^CGAA
AAGC^TT
Methylation sensitivitynot tested
OriginBacillus pumilus 14
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
50 - 7510025 - 5025 - 5075 - 100

References: Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).