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Name Bpu14 I Catalog number E033 E034 Package, u.a. 1000 5000 Concentration, u.a./ml 10000 10000 Recognition site TT^CGAA AAGC^TT Methylation sensitivity not tested Origin Bacillus pumilus 14 Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme % activity: B G O W Y 50 - 75 100 25 - 50 25 - 50 75 - 100 References: Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).