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SibEnzyme Ltd. :: Display product information: Bme18 I
Name
Bme18 I
Catalog numberE029E030
Package, u.a.10005000
Concentration, u.a./ml5000-200005000-20000

Recognition siteG^GWCC
CCWG^G
Methylation sensitivityCleaved of DNA is impaired by overlapping dcm-methylation (CmCWGG): GGWCCWGG.
OriginBacillus megaterium 18
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2525 - 5010075 - 10010 - 25

References: Degtyarev S.K., Rechkunova N.I., Grinev A.A., Dedkov V.S. Izv. Sib. Otd. Akad. Nauk SSSR 15: 25-26 (1989).