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SibEnzyme Ltd. :: Display product information: Bgl II
Name
Bgl II
Catalog numberE027E028
Package, u.a.10005000
Concentration, u.a./ml1000010000

Recognition siteA^GATCT
TCTAG^A
Methylation sensitivityNot blocked by overlapping dam methylation (GmATC) AGATCT
OriginIsolated from an E.coli strain that carries the cloned Bgl II gene from Bacillus globigii
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1010 - 2510025 - 5010 - 25

References: Pirrotta, V. Nucleic Acids Res. 3: 1747-1760 (1976).