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SibEnzyme Ltd. :: Display product information: Bgl I
Bgl I
Catalog numberE025E026
Package, u.a.10005000
Concentration, u.a./ml5000-200005000-20000

Recognition siteGCCNNNN^NGGC
Methylation sensitivityNot blocked by dcm methylation (CmCWGG): GCCWGGNNGGC
OriginBacillus globigii
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-buffer2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2;; 200 mM NaCl; 1 mM DTT)

Enzyme % activity:
50 - 7550 - 750 - 1075 - 10025 - 50

Note: High enzyme concentration may result in star activity
References: Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).