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Name Bgl I Catalog number E025 E026 Package, u.a. 1000 5000 Concentration, u.a./ml 5000-20000 5000-20000 Recognition site GCCNNNN^NGGC CGGN^NNNNCCG Methylation sensitivity Not blocked by dcm methylation (CmCWGG): GCCWGGNNGGC Origin Bacillus globigii Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol. Store at -20°C. Assayed on Lambda DNA Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer 2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2;; 200 mM NaCl; 1 mM DTT) Enzyme % activity: B G O W Y 50 - 75 50 - 75 0 - 10 75 - 100 25 - 50 Note: High enzyme concentration may result in star activity References: Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).