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SibEnzyme Ltd. :: Display product information: Bbv12 I
Name
Bbv12 I
Catalog numberE023E024
Package, u.a.2001000
Concentration, u.a./ml1000-50001000-5000

Recognition siteGWGCW^C
C^WCGWG
Methylation sensitivitynot tested
OriginBacillus brevis 12
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes80oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
0 - 1010 - 2510075 - 10010 - 25

References: Repin, V.E., Rechkunova, N.I., Degtyarev, S.Kh., Hachaturyan, A.A., Afrikyan, E.K. Biol. J. Armenia 42: 969-972 (1989).