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SibEnzyme Ltd. :: Display product information: BamH I
Name
BamH I
Catalog numberE021E022E021XE022X
Package, u.a.400020000400020000
Concentration, u.a./ml20000200005000050000

Recognition siteG^GATCC
CCTAG^G
Methylation sensitivityNot blocked by Dam methylation (GmATC) GGATCC
OriginE.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20C.
Assayed onLambda DNA
LigationAfter 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
25 - 5010075 - 10075 - 10025 - 50

Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975). Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)