|Catalog number||E021||E022||E021X||E022X |
|Package, u.a.||4000||20000||4000||20000 |
|Concentration, u.a./ml||20000||20000||50000||50000 |
|Methylation sensitivity||Not blocked by Dam methylation (GmATC) GGATCC|
|Origin||E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H|
|Storage conditions||10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.|
|Assayed on||Lambda DNA|
|Ligation||After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific activity||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.|
|Optimum temperature||37 oC|
|Inactivation 20 minutes||65oC|
|Optimum SE-buffer||G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA|
Enzyme % activity:
|25 - 50||100||75 - 100||75 - 100||25 - 50|
Note: High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).
Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)