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SibEnzyme Ltd. :: Display product information: AsuNH I
Name
AsuNH I
Catalog numberE063E064
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition siteG^CTAGC
CGATC^G
Methylation sensitivitynot tested
OriginActinobacillus suis NH
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20C
Assayed onLambda DNA (HindIII-digest)
LigationAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific activityNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37C
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA

Enzyme % activity:
BGOWY
75 - 10050 - 750 - 100 - 10100

Note: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
References: Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).