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SibEnzyme Ltd. :: Display product information: AsuHP I
Name
AsuHP I
Catalog numberE231E232
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition siteGGTGA(N)8^
CCACT(N)7^
Methylation sensitivityBlocked by overlapping dam-methylation (GmATC): GGTGATC
OriginActinobacillus suis HP
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 ug/ml BSA; 50% glycerol; Store at -20C.
Assayed onLambda DNA (dam-)
LigationAfter 2-fold overdigestion with enzyme about 30% of the DNA fragments can be ligated and recut.
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

Enzyme % activity:
BGOWY
10 - 2550 - 7510075 - 10025 - 50

Note: Enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
References: Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).