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Name AsuC2 I Catalog number E257 E258 Package, u.a. 2000 10000 Concentration, u.a./ml 20000-50000 20000-50000 Recognition site CC^SGG GGS^CC Methylation sensitivity not tested Origin Actinobacillus suis CA Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Assayed on Lambda DNA Ligation After 5-fold overdigestion less than 10% of the DNA fragments can be ligated Non-specific activity No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 50 u.a. of enzyme for 16 hours at 37°C. Optimum temperature 37 oC Inactivation 20 minutes 65oC Optimum SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme % activity: B G O W Y 75 - 100 50 - 75 10 - 25 25 - 50 100 References: Kileva, E.V., Dedkov, V.S., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1996).