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SibEnzyme Ltd. :: Display product information: AsuC2 I
Name
AsuC2 I
Catalog numberE257E258
Package, u.a.200010000
Concentration, u.a./ml20000-5000020000-50000

Recognition siteCC^SGG
GGS^CC
Methylation sensitivitynot tested
OriginActinobacillus suis CA
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20C.
Assayed onLambda DNA
LigationAfter 5-fold overdigestion less than 10% of the DNA fragments can be ligated
Non-specific activityNo nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 50 u.a. of enzyme for 16 hours at 37C.
Optimum temperature37 oC
Inactivation 20 minutes65oC
Optimum SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme % activity:
BGOWY
75 - 10050 - 7510 - 2525 - 50100

References: Kileva, E.V., Dedkov, V.S., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1996).